3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (C-Term) antibody

Antigen: 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1)

Synonyms: PDK1, PRO0461, MGC20087, MGC35290, Pdk1, PDPK1, pdpk1, MGC82080, B29, IGB, Igb, Igbeta, Ig-beta, MGC108607, CD79B, LOC100304995

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391043

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: PDK1 (PDPK1) (C-term)

Swiss-Prot: NP_002604, O15530

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PDPK1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: 3-phosphoinositide dependent protein kinase-1, hPDK1, PDK1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PDPK1.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

PDPK1 Antibody (C536) (ABIN391043) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PDPK1 antibody detected the PDPK1 protein (arrow).