PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 265-295)

Details for Product anti-PRKAA2 Antibody No. ABIN391058, Supplier: Log in to see
Antigen
  • ampk
  • ampk2
  • prkaa
  • prkaa1
  • PRKAA2
  • AMPK
  • AMPK2
  • AMPKa2
  • PRKAA
  • Ampk
  • Ampka2
  • 2310008I11Rik
  • A830082D05
  • AMPKalpha2
  • AMPKA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
  • prkaa2
  • PRKAA2
  • Prkaa2
Alternatives
anti-Mouse (Murine) PRKAA2 antibody for ELISA
Epitope
AA 265-295, Center
42
16
16
15
15
15
15
14
12
7
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4
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3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
238
128
100
14
10
6
6
5
5
3
3
3
2
2
2
2
2
Host
Rabbit
200
24
19
1
Clonality (Clone)
Polyclonal ()
Conjugate
This PRKAA2 antibody is un-conjugated
12
11
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
183
104
61
39
31
22
18
16
7
4
3
2
1
1
1
1
Options
Supplier
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Immunogen This AMPK gamma (PRKAG1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 AA from the Central region of human AMPK gamma (PRKAG1).
Clone RB3173
Isotype Ig
Specificity This PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273~303 amino acids from the center region of human PRKAG1.
Predicted Reactivity Cow (Bovine),Pig (Porcine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name AMPK gamma (PRKAG1) (PRKAA2 Antibody Abstract)
Background PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.
Synonyms: 5'-AMP-activated protein kinase, gamma-1 subunit, AMPK gamma-1 chain, AMPKg
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Pathways AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 265-295) (ABIN391058) The anti-PRKAG1 Pab (ABIN391058) is used in Western blot to detect PRKAG1 in mouse he...
Immunohistochemistry (IHC) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 265-295) (ABIN391058) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Immunohistochemistry (IHC) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 265-295) (ABIN391058) Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKAG1 Antibody...
Background publications Minokoshi, Alquier, Furukawa, Kim, Lee, Xue, Mu, Foufelle, Ferré, Birnbaum, Stuck, Kahn: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).

Hamilton, Stapleton, ODonnell, Kung, Dalal, Kemp, Witters: "An activating mutation in the gamma1 subunit of the AMP-activated protein kinase." in: FEBS letters, Vol. 500, Issue 3, pp. 163-8, 2001 (PubMed).

Stapleton, Woollatt, Mitchelhill, Nicholl, Fernandez, Michell, Witters, Power, Sutherland, Kemp: "AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location." in: FEBS letters, Vol. 409, Issue 3, pp. 452-6, 1997 (PubMed).