PRKAG3 antibody (AA 148-178)

Catalog No. ABIN391060

Product Details anti-PRKAG3 Antibody

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Binding Specificity: AA 148-178

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-178 amino acids from the Central region of human PRKAG3.

Clone: RB3191

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PRKAG3 antibody (ABIN391060)

Okoshi, Ohta, Ishikawa, Sato, Kizaki: "Expression of 5'-AMP-activated protein kinase with starvation in murine thymocytes." in: The Bulletin of Tokyo Dental College, Vol. 52, Issue 1, pp. 21-9, (2011) (PubMed).

Fukuyama, Ohta, Okoshi, Suehara, Kizaki, Nakagawa: "Hypoxia induces expression and activation of AMPK in rat dental pulp cells." in: Journal of dental research, Vol. 86, Issue 9, pp. 903-7, (2007) (PubMed).

Target Details for PRKAG3

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Alternative Name: PRKAG3 (PRKAG3 Products)

Synonyms: Ampkg3 antibody, prkag3-a antibody, PRKAG3 antibody, AMPKG3 antibody, AMPKg3L antibody, AMPKg3S antibody, Amkg3 antibody, protein kinase AMP-activated non-catalytic subunit gamma 3 antibody, protein kinase, AMP-activated, gamma 3 non-catalytic subunit S homeolog antibody, protein kinase, AMP-activated, gamma 3 non-catatlytic subunit b antibody, protein kinase, AMP-activated, gamma 3 non-catatlytic subunit antibody, Prkag3 antibody, PRKAG3 antibody, prkag3.S antibody, prkag3-b antibody

Background: PRKAG3 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Molecular Weight: 54258

Gene ID: 53632

NCBI Accession: NP_059127

UniProt: Q9UGI9

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Warburg Effect