PGK1 antibody (AA 117-145)

Catalog No. ABIN391135

Product Details anti-PGK1 Antibody

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Binding Specificity: AA 117-145

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity: Hs, Pr, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.

Clone: RB05463

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PGK1 antibody (ABIN391135)

Chen, Liu, Wu, Sheu: "Antrodia cinnamomea Inhibits Migration in Human Hepatocellular Carcinoma Cells: The Role of ERp57 and PGK-1." in: The American journal of Chinese medicine, Vol. 43, Issue 8, pp. 1671-96, (2016) (PubMed).

Ho, Morrison, Silva, Coomber: "The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression." in: Bioscience reports, Vol. 36, Issue 1, pp. e00299, (2016) (PubMed).

Xu, Fang, Zhu, Dai, Nie, Chen, Qin, Wang, Wang, Qiao: "Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis." in: Biology of reproduction, Vol. 89, Issue 6, pp. 142, (2013) (PubMed).

Danshina, Geyer, Dai, Goulding, Willis, Kitto, McCarrey, Eddy, OBrien: "Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice." in: Biology of reproduction, Vol. 82, Issue 1, pp. 136-45, (2009) (PubMed).

Doran, OConnell, Gannon, Kavanagh, Ohlendieck: "Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysis." in: Proteomics, Vol. 8, Issue 2, pp. 364-77, (2008) (PubMed).

Kilic, Kasperczyk, Fulda, Debatin: "Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance." in: Oncogene, Vol. 26, Issue 14, pp. 2027-38, (2007) (PubMed).

OConnell, Gannon, Doran, Ohlendieck: "Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle." in: International journal of molecular medicine, Vol. 20, Issue 2, pp. 145-53, (2007) (PubMed).

West, Moore, Biskup, Bugayenko, Smith, Ross, Dawson, Dawson: "Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 46, pp. 16842-7, (2005) (PubMed).

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1 (PGK1 Products)

Background: Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

Molecular Weight: 44615

Gene ID: 5230

NCBI Accession: NP_000282

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process