Leucine-Rich Repeat Kinase 2 (LRRK2) (Glu519) antibody

Antigen: Leucine-Rich Repeat Kinase 2 (LRRK2)

Synonyms: Gm927, cI-46, AW561911, 4921513O20Rik, 9330188B09Rik, D630001M17Rik, PARK8, RIPK7, ROCO2, AURA17, DARDARIN, LRRK2

Binding Site: Glu519

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391145

Additional Information

Alternative name: PARK8 (LRRK2)

Gene ID: 120892

UniProt: NP_940980

Immunogen: This PARK8 (LRRK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid E519 of human LRRK2.

Isotype: Ig

Description: Other names: Park8, Leucine-rich repeat (LRR) kinase 2, Leucine-rich repeat kinase 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PARK8 (LRRK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid E519 of human LRRK2.

Molecular Weight: 280863 DA

Comments:

Background: Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson's disease identified to date. LRRK2, a genetic mutation, was recently found linked to about 5 percent of inherited cases of Parkinson's disease. By high-resolution recombination mapping and candidate gene sequencing in 46 families, 6 disease-segregating mutations (5 missense and 1 putative splice site mutation). It may be central to the pathogenesis of several major neurodegenerative disorders associated with parkinsonism. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 WB~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Fresh frozen tissue samples from the human temporal lobe (Htl) is subjected to different extraction buffers(Htl/Low Salt, Htl/high salt, Htl/1%triton, Htl/RIPA, Htl/1%SDS, HEK293T-pcDNA3, HEK293T-hLRRK2). PARK8 (LRRK2) Antibody (E519)(ABIN391145) has sufficient specificity to detect endogenous human LRRK2.

PARK8 (LRRK2) Antibody (E519)(ABIN391145) detect over-expressed human LRRK2 protein.

Publications

Product: Shen: "Protein kinases linked to the pathogenesis of Parkinson's disease." in: Neuron, Vol. 44, Issue 4, pp. 575-7, 2004 (PubMed).

Paisán-Ruíz, Jain, Evans et al.: "Cloning of the gene containing mutations that cause PARK8-linked Parkinson's disease." in: Neuron, Vol. 44, Issue 4, pp. 595-600, 2004 (PubMed).

Zimprich, Biskup, Leitner et al.: "Mutations in LRRK2 cause autosomal-dominant parkinsonism with pleomorphic pathology." in: Neuron, Vol. 44, Issue 4, pp. 601-7, 2004 (PubMed).