Anti-Mullerian Hormone Receptor, Type II (AMHR2) (C-Term) antibody

Antigen: Anti-Mullerian Hormone Receptor, Type II (AMHR2)

Synonyms: AMHR, MISR2, MISRII, Mrii, Misiir, Misrii, MGC141312, AMHR2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391175

Additional Information

Alternative name: AMHR2

Gene ID: 269

UniProt: Q16671

Immunogen: This AMHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381~410 amino acids from the N-terminal region of human AMHR2.

Isotype: Ig

Description: Other names: Anti-Muellerian hormone type II receptor [Precursor], AMH type II receptor, MIS type II receptor, MISRII, MRII, AMHR2, AMHR,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AMHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381~410 amino acids from the N-terminal region of human AMHR2.

Molecular Weight: 62750 DA

Comments:

Background: The AMH receptor (AMHR or AMHR2) is a serine/threonine kinase with a single transmembrane domain belonging to the family of type II receptors for TGF-beta-related proteins. Anti-Mullerian hormone (AMH) and its receptor are involved in the regression of Mullerian ducts in male fetuses. Male sex differentiation is mediated by 2 discrete hormones produced by the fetal testis. Testosterone, produced by Leydig cells, virilizes the external genitalia and promotes prostatic growth, anti-Mullerian hormone (AMH) results in regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AMHR2 Antibody (C-term) (ABIN391175) in CEM cell line lysates (35ug/lane).AMHR2 (arrow) was detected using the purified Pab.

Western blot analysis of AMHR2 (arrow) using AMHR2 Antibody (C-term) (ABIN391175). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMHR2 gene (Lane 2) (Origene Technologies).

Flow cytometric analysis of CEM cells using AMHR2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Visser, McLuskey, van Beers et al.: "Structure and chromosomal localization of the human anti-müllerian hormone type II receptor gene." in: Biochemical and biophysical research communications, Vol. 215, Issue 3, pp. 1029-36, 1995 (PubMed).