Fas-Activated serine/threonine Kinase (FASTK) (Center) antibody

Antigen: Fas-Activated serine/threonine Kinase (FASTK)

Synonyms: FAST, 610011K02Rik, 0610011K02Rik, FASTK, MGC137976, DKFZp469E0832

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391196

Additional Information

Alternative name: FASTK

Gene ID: 10922

UniProt: Q14296

Immunogen: This FASTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~246 amino acids from the center region of human FASTK.

Isotype: Ig

Description: Other names: Fas-activated serine/threonine kinase, FAST kinase, FASTK,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FASTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~246 amino acids from the center region of human FASTK.

Molecular Weight: 61104 DA

Comments:

Background: FASTK is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-FASTK (ABIN391196) in Hela cell line lysate (35ug/lane). FASTK(arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of FASTK (arrow) using rabbit polyclonal FASTK Antibody (Center) (ABIN391196). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FASTK gene.

Publications

Product: Scherer, Cheung, MacDonald et al.: "Human chromosome 7: DNA sequence and biology." in: Science (New York, N.Y.), Vol. 300, Issue 5620, pp. 767-72, 2003 (PubMed).

Li, Simarro, Kedersha et al.: "FAST is a survival protein that senses mitochondrial stress and modulates TIA-1-regulated changes in protein expression." in: Molecular and cellular biology, Vol. 24, Issue 24, pp. 10718-32, 2004 (PubMed).