Heat Shock 22kDa Protein 8 (HSPB8) (N-Term) antibody

Antigen: Heat Shock 22kDa Protein 8 (HSPB8)

Synonyms: H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, MGC64408, HSPB8, DKFZp468F234, fc09c11, MGC64202, zgc:64202, wu:fc04b04, wu:fc09c11

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391207

Additional Information

Alternative name: Hsp22 (HSPB8)

Gene ID: 26353

UniProt: Q9UJY1

Immunogen: This Hsp22 (HSPB8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids from the N-terminal region of human HSPB8.

Isotype: Ig

Description: Other names: Heat-shock protein beta-8, HspB8, Alpha crystallin C chain, Small stress protein-like protein HSP22, E2-induced gene 1 protein, Protein kinase H11, HSPB8, CRYAC, E2IG1, HSP22,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Hsp22 (HSPB8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids from the N-terminal region of human HSPB8.

Molecular Weight: 21604 DA

Comments:

Background: Heat-shock protein beta-8 (HSPB8) displays temperature-dependent chaperone activity. It acts as a Mn(2+)-dependent serine-threonine-specific protein kinase. Defects in HSPB8 are a cause of distal hereditary motor neuropathy type II , also known as distal spinal muscular atrophy and spinal muscular atrophy of the Charcot-Marie-Tooth type. It is also an autosomal dominant disorder of lower motor neurons characterized by distal muscle weakness.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-HSPB8 Pab (ABIN391207) is used in Western blot to detect HSPB8 in mouse heart tissue lysate

Publications

Product: Kappé, Verschuure, Philipsen et al.: "Characterization of two novel human small heat shock proteins: protein kinase-related HspB8 and testis-specific HspB9." in: Biochimica et biophysica acta, Vol. 1520, Issue 1, pp. 1-6, 2001 (PubMed).

Gober, Smith, Ueda et al.: "Forced expression of the H11 heat shock protein can be regulated by DNA methylation and trigger apoptosis in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37600-9, 2003 (PubMed).

Irobi, Van Impe, Seeman et al.: "Hot-spot residue in small heat-shock protein 22 causes distal motor neuropathy." in: Nature genetics, Vol. 36, Issue 6, pp. 597-601, 2004 (PubMed).