MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term), (AA 6-40) antibody

Details for Product No. ABIN391219
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Antigen
Synonyms MGC80341, MARK1, AW491150, B930025N23Rik, Emk3, Par-1c, mKIAA1477, mPar-1c, MARK, Par1c
Epitope
N-Term, AA 6-40
(23), (15), (7), (7), (6), (6), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(55), (19), (10), (1)
Host
Rabbit
(57), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(50), (41), (13), (12), (6), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN391219
291.50 $
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Immunogen This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-40 AA from the N-terminal region of human MARK1.
Clone RB8545
Isotype Ig
Specificity This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of human MARK1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MARK1
Background MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK
Molecular Weight 85000 DA
Gene ID 4139
UniProt Q2HIY1
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term), (AA 6-40) antibody The anti-MARK1 N-term Pab (ABIN391219) is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term), (AA 6-40) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term) (ABIN391219), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Drewes, Ebneth, Preuss et al.: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, 1997 (PubMed).

Validation Images
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