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MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (AA 525-554)

Details for Product anti-MELK Antibody No. ABIN391224, Supplier: Log in to see
Antigen
  • MELK
  • xmelk
  • Melk
  • HPK38
  • AI327312
  • MPK38
  • mKIAA0175
  • fb71b01
  • fe01f04
  • wu:fb71b01
  • wu:fe01f04
Epitope
AA 525-554, C-Term
40
18
13
13
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
109
27
18
6
6
2
2
2
2
2
Host
Rabbit
86
20
8
Clonality (Clone)
Polyclonal ()
Conjugate
This MELK antibody is un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
85
60
11
8
7
5
5
5
3
2
1
Supplier
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Immunogen This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525-554 AA from the C-terminal region of human MELK.
Clone RB8557
Isotype Ig
Specificity This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532~562 amino acids from the C-terminal region of human MELK.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MELK (MELK Antibody Abstract)
Background MELK Contains 1 protein kinase domain that Belongs to the Ser/Thr protein kinase family. It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may also be involved in the inhibition of spliceosome assembly during mitosis.
Synonyms: Maternal embryonic leucine zipper kinase, hMELK, Protein kinase PK38, hPK38, MELK, KIAA0175
Molecular Weight 74642 DA
Gene ID 9833
UniProt Q14680
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (AA 525-554) (ABIN391224) The anti-MELK Pab (ABIN391224) is used in Western blot to detect MKEL in HEPG2 tissue...
Background publications Davezac, Baldin, Blot et al.: "Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation." in: Oncogene, Vol. 21, Issue 50, pp. 7630-41, 2002 (PubMed).