SEPSH2 (SEPSH2) (AA 171-201), (Center) antibody

Details for Product No. ABIN391279
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Antigen
Epitope
AA 171-201, Center
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN391279
Quantity 400 µL
Price
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Immunogen This SEPSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-201 AA from the Central region of human SEPSH2.
Clone RB5623
Isotype Ig
Specificity This SEPSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179~208 amino acids from the Center region of human SEPSH2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SEPSH2
Background This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
Synonyms: Selenide, water dikinase 2, Selenophosphate synthetase 2, Selenium donor protein 2, SPS2
Molecular Weight 47258 DA
Gene ID 22928
UniProt Q99611
Research Area Translation Factors, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SEPSH2 (SEPSH2) (AA 171-201), (Center) antibody Western blot analysis of anti-SEPSH2 Pab(ABIN391279) in HepG2 cell line lysate (35 µg/lane). SEPSH2(arrow) was detected using the purified polyclonal antibody (1:50 dilution).
anti-SEPSH2 (SEPSH2) (AA 171-201), (Center) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Guimarães, Peterson, Vicari et al.: "Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?" in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 26, pp. 15086-91, 1997 (PubMed).

Lescure, Gautheret, Carbon et al.: "Novel selenoproteins identified in silico and in vivo by using a conserved RNA structural motif." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 38147-54, 2000 (PubMed).

Tamura, Yamamoto, Takahata et al.: "Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16162-7, 2004 (PubMed).

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