serine/threonine Kinase 17b (STK17B) (C-Term) antibody

Antigen: serine/threonine Kinase 17b (STK17B)

Synonyms: DRAK2, Drak2, MGC65989, zgc:65989, STK17B, MGC134048

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391326

Additional Information

Alternative name: DRAK2

Gene ID: 9262

UniProt: O94768

Immunogen: This DRAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human DRAK2.

Isotype: Ig

Description: Other names: Serine/threonine protein kinase 17B, DAP kinase-related apoptosis-inducing protein kinase 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DRAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human DRAK2.

Molecular Weight: 42344 DA

Comments:

Background: DRAK2 is a novel serine/threonine kinase that induces apoptosis via catalytic activity. DRAKs present high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases. DRAK2 is located in nucleus, and the messenger RNA is ubiquitously expressed in human tissues.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-DRAK2 Pab (ABIN391326) is used in Western blot to detect DRAK2 in mouse lung tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

IF staining of Drak2 in WT versus Tg T cells. Permeablized lymph node T cells were double-stained with rabbit anti-Drak2/sheep anti-rabbit Ig-FITC and anti-Thy1.2-PE monoclonal antibody, and signals were registered by confocal microscopy. THE cell surface Thy1.2 is shown in red, and intracellular Drak2 is in green.

Publications

Product: Sanjo, Kawai, Akira: "DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 29066-71, 1998 (PubMed).