Mitogen-Activated Protein Kinase 8 (MAPK8) (Center) antibody

Antigen: Mitogen-Activated Protein Kinase 8 (MAPK8)

Synonyms: JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2, Prkm8, AI849689, Bsk, D-JNK, D-junk, DBSK/JNK, DJNK, DJNK/bsk, JNK/SAPK, Junk, SAPKa, dJUN, DmelCG5680, CG5680, T10F20.15

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391335

Additional Information

Alternative name: JNK1 (MAPK8)

Gene ID: 5599

UniProt: P45983

Immunogen: This JNK1 (MAPK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269~299 amino acids from the center region of human MAPK8.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JNK1 (MAPK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269~299 amino acids from the center region of human MAPK8.

Molecular Weight: 48295 DA

Comments:

Background: MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-MAPK8 Pab (ABIN391335) in mouse spleen tissue lysate. MAPK8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of anti-MAPK8 Antibody (Center) (ABIN391335) in Jurkat cell line lysates (35ug/lane). MAPK8 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Vivo, Liu, Broaddus: "c-Jun N-terminal kinase contributes to apoptotic synergy induced by tumor necrosis factor-related apoptosis-inducing ligand plus DNA damage in chemoresistant, p53 inactive mesothelioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25461-7, 2003 (PubMed).

Utsugi, Dobashi, Ishizuka et al.: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 628-35, 2003 (PubMed).

Ghosh: "Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase." in: Biochemical and biophysical research communications, Vol. 307, Issue 2, pp. 342-9, 2003 (PubMed).

Luo, Ng, Jin et al.: "Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37451-8, 2003 (PubMed).

Deng, Ren, Yang et al.: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).