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Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (AA 361-390), (C-Term) antibody

Details for Product No. ABIN391394, Supplier: Login to see
Antigen
  • DKFZp459H2130
  • 2210402P09Rik
  • AI844555
  • CAL
  • FIG
  • GOPC1
  • PIST
  • fe38f01
  • wu:fe38f01
  • zgc:56254
  • zgc:65853
  • dJ94G16.2
  • cal
  • gopc1
  • pist
Epitope
AA 361-390, C-Term
59
19
15
11
4
4
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
132
42
34
4
4
1
1
Host
Rabbit
117
15
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
8
8
8
7
7
7
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
114
70
67
16
14
11
5
2
2
1
1
Supplier
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Immunogen This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 AA from the C-terminal region of human PIST.
Clone RB13073
Isotype Ig
Specificity This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368~398 amino acids from the C-terminal region of human PIST.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PIST (GOPC Antibody Abstract)
Background PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Synonyms: PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma
Molecular Weight 50389 DA
Gene ID 57120
UniProt Q9HD26
Research Area Protein Modifications, Autophagy, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (AA 361-390), (C-Term) antibody (ABIN391394) Western blot analysis of PIST Antibody (C-term) (ABIN391394) in Ramos cell line lysat...
Immunohistochemistry (IHC) image for anti-Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (AA 361-390), (C-Term) antibody (ABIN391394) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST a...
Product cited in: Hnasko, Lin, McGarvey et al.: "A rapid method to improve protein detection by indirect ELISA." in: Biochemical and biophysical research communications, Vol. 410, Issue 4, pp. 726-31, 2011 (PubMed).

Background publications Wente, Stroh, Beaudet et al.: "Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5." in: The Journal of biological chemistry, Vol. 280, Issue 37, pp. 32419-25, 2005 (PubMed).