beta 2 Adrenergic Receptor (BAR2) (N-Term) antibody

Antigen: beta 2 Adrenergic Receptor (BAR2)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391396

Additional Information

Gene ID: 154

UniProt: NP_000015

Immunogen: This BAR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human BAR2.

Isotype: Ig

Description: Other names: Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, ADRB2R, B2AR

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BAR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human BAR2.

Molecular Weight: 46426 DA

Comments:

Background: This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting ~~ 1:100~500 Immunohistochemistry ~~ Not Tested Immunoprecipitation ~~ Not tested Flow cytometry ~~ Not tested

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-BAR2 Antibody (N-term) (ABIN391396) in HL60 cell line lysates (35ug/lane). BAR2(arrow) was detected using the purified Pab (1:440 dilution).

Publications

Rodrigues, Tran, Fortes et al.: "Matrix Metalloproteinases Cleave the {beta}2 -Adrenergic Receptor in the Spontaneously Hypertensive Rats." in: American journal of physiology. Heart and circulatory physiology, 2010 (PubMed).

Product: Cherezov, Rosenbaum, Hanson et al.: "High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 318, Issue 5854, pp. 1258-65, 2007 (PubMed).