Carbonic Anhydrase XI (CA11) (Center) antibody

Antigen: Carbonic Anhydrase XI (CA11)

Synonyms: CARPX1, CAR-XI, MGC93050, CAR11, MGC143020

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391472

Additional Information

Alternative name: CA11

Gene ID: 770

UniProt: O75493

Immunogen: This CA11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193~223 amino acids from the Center region of human CA11.

Isotype: Ig

Description: Other names: Carbonic anhydrase-related protein 11, CA-RP XI, CARP XI, CA-XI, Carbonic anhydrase-related protein 2, CARP-2, CA-RP II, UNQ211/PRO237, CARP2, CA11

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CA11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193~223 amino acids from the Center region of human CA11.

Molecular Weight: 36238 DA

Comments:

Background: CA11 belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CA11 is likely a secreted protein, however, radical changes at active site residues completely conserved in CA isozymes with catalytic activity, make it unlikely that it has carbonic anhydrase activity. This protein shares properties in common with two other acatalytic CA isoforms, CA VIII and CA X. The protein is most abundantly expressed in brain, and may play a general role in the central nervous system.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CA11 antibody (Center) (ABIN391472) in K562 cell line lysates (35ug/lane). CA11 (arrow) was detected using the purified Pab.

Western blot analysis of CA11 antibody (Center) (ABIN391472) in mouse brain tissue lysates (35ug/lane). CA11 (arrow) was detected using the purified Pab.

Publications

Product: Lovejoy, Hewett-Emmett, Porter et al.: "Evolutionarily conserved, "acatalytic" carbonic anhydrase-related protein XI contains a sequence motif present in the neuropeptide sauvagine: the human CA-RP XI gene (CA11) is embedded between the secretor gene cluster and the DBP gene at 19q13.3." in: Genomics, Vol. 54, Issue 3, pp. 484-93, 1999 (PubMed).

Bellingham, Gregory-Evans, Gregory-Evans: "Sequence and tissue expression of a novel human carbonic anhydrase-related protein, CARP-2, mapping to chromosome 19q13.3." in: Biochemical and biophysical research communications, Vol. 253, Issue 2, pp. 364-7, 1999 (PubMed).