|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Checkpoint Kinase 1 (CHEK1) (C-Term) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~462 amino acids from the C-terminal region of human CHK1.|
|Description||Other names: Serine/threonine-protein kinase Chk1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~462 amino acids from the C-terminal region of human CHK1.|
|Molecular Weight||54419 DA|
Background: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Xiao, Chen, Gunasekera et al.: "Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21767-73, 2003 (PubMed).
Goudelock, Jiang, Pereira et al.: "Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29940-7, 2003 (PubMed).
Wang, Khadpe, Hu et al.: "An overactivated ATR/CHK1 pathway is responsible for the prolonged G2 accumulation in irradiated AT cells." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30869-74, 2003 (PubMed).