Checkpoint Kinase 1 (CHEK1) (C-Term) antibody

Antigen: Checkpoint Kinase 1 (CHEK1)

Synonyms: CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391598

Additional Information

Alternative name: CHK1

Gene ID: 1111

UniProt: O14757

Immunogen: This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~462 amino acids from the C-terminal region of human CHK1.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Chk1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~462 amino acids from the C-terminal region of human CHK1.

Molecular Weight: 54419 DA

Comments:

Background: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CHK1 C-term Pab (ABIN391598) in mouse muscle tissue lysate (lane A) and in HL-60 cell lysate (lane B). CHK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Xiao, Chen, Gunasekera et al.: "Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21767-73, 2003 (PubMed).

Goudelock, Jiang, Pereira et al.: "Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29940-7, 2003 (PubMed).

Wang, Khadpe, Hu et al.: "An overactivated ATR/CHK1 pathway is responsible for the prolonged G2 accumulation in irradiated AT cells." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30869-74, 2003 (PubMed).