Checkpoint Kinase 2 (CHEK2) (C-Term) antibody

Antigen: Checkpoint Kinase 2 (CHEK2)

Synonyms: CDS1, CHK2, LFS2, RAD53, HuCds1, PP1425

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391604

Additional Information

Alternative name: CHK2

Gene ID: 11200

UniProt: O96017

Immunogen: This CHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CHK2.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Chk2, Cds1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CHK2.

Molecular Weight: 60914 DA

Comments:

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this protein are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CHK2 C-term Pab (ABIN391604) in HL-60 cell lysate. CHK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Louria-Hayon, Grossman, Sionov et al.: "The promyelocytic leukemia protein protects p53 from Mdm2-mediated inhibition and degradation." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33134-41, 2003 (PubMed).

Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003 (PubMed).

Tan, Guschin, Davalos et al.: "Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 21, pp. 11997-2002, 2003 (PubMed).