Vaccinia Related Kinase 2 (VRK2) (N-Term) antibody

Antigen: Vaccinia Related Kinase 2 (VRK2)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391606

Additional Information

Alternative name: VRK2

Gene ID: 7444

UniProt: Q99987

Immunogen: This VRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human VRK2.

Isotype: Ig

Description: Other names: Vaccinia related kinase 2, Serine/threonine-protein kinase VRK2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human VRK2.

Molecular Weight: 58126 DA

Comments:

Background: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. While several transcript variants may exist for this gene, the full-length nature of only one has been biologically validated to date.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-VRK2 Antibody (N-term) (ABIN391606) in K562 cell line lysates (35ug/lane). VRK2(arrow) was detected using the purified Pab.

Publications

Product: Nezu, Oku, Jones et al.: "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase." in: Genomics, Vol. 45, Issue 2, pp. 327-31, 1998 (PubMed).