ADAM Metallopeptidase Domain 15 (ADAM15) (Center) antibody

Antigen: ADAM Metallopeptidase Domain 15 (ADAM15)

Synonyms: MDC15, metargidin, tMDCVI, ADAM15, mdc15

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391624

Additional Information

Alternative name: ADAM15

Gene ID: 8751

UniProt: Q13444

Immunogen: This ADAM15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214~243 amino acids from the center region of human ADAM15.

Isotype: Ig

Description: Other names: Metargidin, Metalloprotease RGD disintegrin protein, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, MDC-15, A disintegrin and metalloproteinase domain 15, ADAM 15, ADAM15, MDC15

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADAM15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214~243 amino acids from the center region of human ADAM15.

Molecular Weight: 87717 DA

Comments:

Background: ADAM15 is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, the protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ADAM15 Antibody (Center) (ABIN391624) in NCI-H460 cell line lysates (35ug/lane). ADAM15 (arrow) was detected using the purified Pab.

Publications

Product: McKie, Edwards, Dallas et al.: "Expression of members of a novel membrane linked metalloproteinase family (ADAM) in human articular chondrocytes." in: Biochemical and biophysical research communications, Vol. 230, Issue 2, pp. 335-9, 1997 (PubMed).

Zhang, Kamata, Yokoyama et al.: "Specific interaction of the recombinant disintegrin-like domain of MDC-15 (metargidin, ADAM-15) with integrin alphavbeta3." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7345-50, 1998 (PubMed).

Poghosyan, Robbins, Houslay et al.: "Phosphorylation-dependent interactions between ADAM15 cytoplasmic domain and Src family protein-tyrosine kinases." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4999-5007, 2002 (PubMed).