ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) (Center) antibody

Antigen: ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13)

Synonyms: Gm710, vWF-CP, ADAMTS13, TTP, VWFCP, C9orf8, FLJ42993, MGC118899, MGC118900, DKFZp434C2322, PRO4389, MGC126403, FLJ16363, FLJ32769, AI605170, ADAMTS-12, ADAMTS12

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391641

Additional Information

Alternative name: ADAMTS13

Gene ID: 11093

UniProt: Q76LX8

Immunogen: This ADAMTS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 836~866 amino acids from the center region of human ADAMTS13.

Isotype: Ig

Description: Other names: von Willebrand factor-cleaving protease, vWF-cleaving protease, vWF-CP, A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAMTS-13, ADAM-TS 13, ADAM-TS13, ADAMTS13, C9orf8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADAMTS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 836~866 amino acids from the center region of human ADAMTS13.

Molecular Weight: 153604 DA

Comments:

Background: ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ADAMTS13 Antibody (Center)(ABIN391641) in CEM cell line lysates (35ug/lane). ADAMTS13 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADAMTS13 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Zheng, Chung, Takayama et al.: "Structure of von Willebrand factor-cleaving protease (ADAMTS13), a metalloprotease involved in thrombotic thrombocytopenic purpura." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 41059-63, 2001 (PubMed).

Levy, Nichols, Lian et al.: "Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura." in: Nature, Vol. 413, Issue 6855, pp. 488-94, 2001 (PubMed).

Plaimauer, Fuhrmann, Mohr et al.: "Modulation of ADAMTS13 secretion and specific activity by a combination of common amino acid polymorphisms and a missense mutation." in: Blood, Vol. 107, Issue 1, pp. 118-25, 2005 (PubMed).