Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) (C-Term) antibody

Antigen: Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML)

Synonyms: LY6DL, GML, HemT, EG625599, MGC134423

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391648

Additional Information

Alternative name: GML

Gene ID: 2765

UniProt: Q99445

Immunogen: This GML antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~151 amino acids from the C-terminal region of human GML.

Isotype: Ig

Description: Other names: Glycosyl-phosphatidylinositol-anchored molecule-like protein, GML, LY6DL

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GML antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~151 amino acids from the C-terminal region of human GML.

Molecular Weight: 17730 DA

Comments:

Background: GML may play a role in the apoptotic pathway or cell-cycle regulation induced by p53 after DNA damage.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-GML Antibody (C-term) (ABIN391648) in K562 cell line lysates (35ug/lane). GML(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis reacted with GML Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Furuhata, Tokino, Urano et al.: "Isolation of a novel GPI-anchored gene specifically regulated by p53; correlation between its expression and anti-cancer drug sensitivity." in: Oncogene, Vol. 13, Issue 9, pp. 1965-70, 1997 (PubMed).

Kimura, Furuhata, Urano et al.: "Genomic structure and chromosomal localization of GML (GPI-anchored molecule-like protein), a gene induced by p53." in: Genomics, Vol. 41, Issue 3, pp. 477-80, 1997 (PubMed).