Carbonic Anhydrase VII (CA7) (Center) antibody

Antigen: Carbonic Anhydrase VII (CA7)

Synonyms: CAVII, Ca7, AV343731, MGC73385, MGC174490, zgc:73385

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391692

Additional Information

Alternative name: CA7

Gene ID: 762

UniProt: P22748

Immunogen: This CA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94~124 amino acids from the Center region of human CA7.

Isotype: Ig

Description: Other names: Carbonic anhydrase 4, Carbonic anhydrase IV, CA-IV, Carbonate dehydratase IV , CA4

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94~124 amino acids from the Center region of human CA7.

Molecular Weight: 35032 DA

Comments:

Background: CA7 is a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This protein is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known, however, the protein may have a role in inherited renal abnormalities of bicarbonate transport.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CA7 antibody (Center) (ABIN391692) in mouse heart tissue lysates (35ug/lane). CA7 (arrow) was detected using the purified Pab.

Western blot analysis of CA7 (arrow) using rabbit polyclonal CA7 Antibody (Center) (ABIN391692). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA7 gene.

Publications

Product: Okuyama, Sato, Zhu et al.: "Human carbonic anhydrase IV: cDNA cloning, sequence comparison, and expression in COS cell membranes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1315-9, 1992 (PubMed).

Okuyama, Batanian, Sly: "Genomic organization and localization of gene for human carbonic anhydrase IV to chromosome 17q." in: Genomics, Vol. 16, Issue 3, pp. 678-84, 1993 (PubMed).

Yang, Alvarez, Chakarova et al.: "Mutant carbonic anhydrase 4 impairs pH regulation and causes retinal photoreceptor degeneration." in: Human molecular genetics, Vol. 14, Issue 2, pp. 255-65, 2004 (PubMed).