Capping Protein (Actin Filament) Muscle Z-Line, alpha 1 (CAPZA1) (Center) antibody

Antigen: Capping Protein (Actin Filament) Muscle Z-Line, alpha 1 (CAPZA1)

Synonyms: CAPZ, CAZ1, CAPPA1, Cappa1, MGC68726, capza, capza1, CAPZA1, MGC138080, DKFZp459D0820, zgc:56314, zgc:85654, wu:fa19e05, wu:fk22d04, fb36c09, fc85a01, fj45f05, sb:cb852, zK16K6.1, wu:fb36c09, wu:fc85a01, wu:fj45f05, zgc:101755, capz, caz1, cappa1, MGC76174

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391705

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: CAPZA1 (Center)

Gene ID: 829

Swiss-Prot: P52907

Immunogen: This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82~111 amino acids from the Center region of human CAPZA1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CAPZA1.

Molecular Weight: 32923 DA

Synonyms: CAPZ, CAZ1, CAPPA1, Cappa1, MGC68726, capza, capza1, CAPZA1, MGC138080, DKFZp459D0820, zgc:56314, zgc:85654, wu:fa19e05, wu:fk22d04, fb36c09, fc85a01, fj45f05, sb:cb852, zK16K6.1, wu:fb36c09, wu:fc85a01, wu:fj45f05, zgc:101755, capz, caz1, cappa1, MGC76174

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CAPZA1 antibody (Center) (ABIN391705) in SK-Br-3 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with CAPZA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-231 cells using CAPZA1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.