Mitogen-Activated Protein Kinase 7 (MAPK7) (Thr218,Tyr220) antibody

Antigen: Mitogen-Activated Protein Kinase 7 (MAPK7)

Synonyms: BMK1, ERK4, ERK5, PRKM7, Erk5-T, ATMPK7, F8D23.5, F8D23_5, MAP kinase 7, MPK7

Binding Site: Thr218,Tyr220

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391723

Additional Information

Alternative name: ERK5

Gene ID: 5598

UniProt: Q13164

Immunogen: This ERK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206~236 amino acids surrounding T218 of human ERK5.

Isotype: Ig

Description: Other names: Extracellular signal-regulated kinase 5, Mitogen-activated protein kinase 7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ERK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206~236 amino acids surrounding T218 of human ERK5.

Molecular Weight: 88636 DA

Comments:

Background: MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ERK5 Antibody (T218/Y220) (ABIN391723) in Ramos cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.

ERK5 Antibody (T218/Y220) (ABIN391723) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERK5 Antibody (T218/Y220) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Lee, Ulevitch, Han: "Primary structure of BMK1: a new mammalian map kinase." in: Biochemical and biophysical research communications, Vol. 213, Issue 2, pp. 715-24, 1995 (PubMed).

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995 (PubMed).