Mitogen-Activated Protein Kinase P38 beta (p38-beta) (C-Term,Lys356) antibody

Antigen: Mitogen-Activated Protein Kinase P38 beta (p38-beta)

Binding Site: C-Term,Lys356

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391729

Additional Information

Alternative name: p38 beta

Gene ID: 5600

UniProt: Q15759

Immunogen: This p38 beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human P38beta.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 11, Mitogen-activated protein kinase p38 beta, MAP kinase p38 beta, p38b, p38-2, Stress-activated protein kinase-2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This p38 beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human P38beta.

Molecular Weight: 42132 DA

Comments:

Background: P38 beta is a member of the MAP kinase subfamily of Ser/Thr protein kinases. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by changes in osmolarity of the extracellular environment, proinflammatory cytokines and cellular stress. This protein preferentially phosphorylates transcription factor ATF2. It is activated by phosphorylation on threonine and tyrosine by MKK6, and inhibited by pyridinyl-imidazole related compounds. Highest levels of expression are found in the brain and heart, with additional expression in the placenta, lung, liver, skeletal muscle, kidney and pancreas.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-P38beta C-term Pab (ABIN391729) in placenta lysate. P38beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Kumar, McDonnell, Gum et al.: "Novel homologues of CSBP/p38 MAP kinase: activation, substrate specificity and sensitivity to inhibition by pyridinyl imidazoles." in: Biochemical and biophysical research communications, Vol. 235, Issue 3, pp. 533-8, 1997 (PubMed).

Stein, Yang, Young et al.: "p38-2, a novel mitogen-activated protein kinase with distinct properties." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19509-17, 1997 (PubMed).

Enslen, Raingeaud, Davis: "Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1741-8, 1998 (PubMed).

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).