Cyclin-Dependent Kinase 11B (CDK11B) (N-Term) antibody

Antigen: Cyclin-Dependent Kinase 11B (CDK11B)

Synonyms: p58, PK58, CDK11, CLK-1, CDC2L2, FLJ59152, p58CLK-1, CDK11-p46, CDK11-p58, p58CDC2L1, CDK11-p110

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391736

Additional Information

Alternative name: CDC2L1

Gene ID: 984

UniProt: P21127

Immunogen: This CDC2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human CDC2L1.

Isotype: Ig

Description: Other names: Galactosyltransferase associated protein kinase p58/GTA, Cell division cycle 2-like 1, CLK-1, p58 CLK-1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human CDC2L1.

Molecular Weight: 92707 DA

Comments:

Background: CDC2L1 is a member of the p34Cdc2 protein kinase family. p34Cdc2 kinase family members are known to be essential for eukaryotic cell cycle control. The gene is in close proximity to CDC2L2, a nearly identical gene in the same chromosomal region. The gene loci including this gene, CDC2L2, as well as metalloprotease MMP21/22, consist of two identical, tandemly linked genomic regions which are thought to be a part of the larger region that has been duplicated. The genes for CDC2L1 and CDC2L2 were shown to be deleted or altered frequently in neuroblastoma with amplified MYCN genes. The protein is cleavable by caspases and hs been demonstrated to play roles in cell apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot ~~ 1:50~200 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CDC2L1 Antibody (N-term) (ABIN391736) in HepG2 cell line lysates (35ug/lane). CDC2L1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: White, Maris, Beltinger et al.: "A region of consistent deletion in neuroblastoma maps within human chromosome 1p36.2-36.3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5520-4, 1995 (PubMed).

Cornelis, Bruynooghe, Denecker et al.: "Identification and characterization of a novel cell cycle-regulated internal ribosome entry site." in: Molecular cell, Vol. 5, Issue 4, pp. 597-605, 2000 (PubMed).

Zhang, Cai, Zhang et al.: "Interaction of p58(PITSLRE), a G2/M-specific protein kinase, with cyclin D3." in: The Journal of biological chemistry, Vol. 277, Issue 38, pp. 35314-22, 2002 (PubMed).