Cyclin-Dependent Kinase 11B (CDK11B) (C-Term) antibody

Antigen: Cyclin-Dependent Kinase 11B (CDK11B)

Synonyms: p58, PK58, CDK11, CLK-1, CDC2L2, FLJ59152, p58CLK-1, CDK11-p46, CDK11-p58, p58CDC2L1, CDK11-p110

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391737

Additional Information

Alternative name: CDC2L1

Gene ID: 984

UniProt: P21127

Immunogen: This CDC2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~406 amino acids from the C-terminal region of human CDC2L1.

Isotype: Ig

Description: Other names: Galactosyltransferase associated protein kinase p58/GTA, Cell division cycle 2-like 1, CLK-1, p58 CLK-1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~406 amino acids from the C-terminal region of human CDC2L1.

Molecular Weight: 92707 DA

Comments:

Background: CDC2L1, a member of the CDC2/CDKX subfamily of Ser/Thr protein kinases, appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression. Phosphorylation at Thr-433 or Tyr-434 inactivates the enzyme, while phosphorylation at Thr-580 activates it The cleaved p110 isoform, p110C, binds to the serine/threonine kinase PAK1. The p58 isoform but not the p110 isoform or p110C interacts with CCND3. The p110 isoforms are found in large molecular weight complexes containing CCNL1 and SFRS7. This protein is expressed ubiquitously, although there is some evidence of isoform-specific tissue distribution. The p58 isoform is specifically induced in G2/M phase of the cell cycle. During apoptosis, induced by Fas or tumor necrosis factor, specific PITSLRE p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the PITSLRE proteins. CDC2L1 and CDC2L2 encode almost identical protein kinases of 110 kDa that contain at their C termini the open reading frame of a smaller 58 kDa isoform which is expressed following IRES-mediated alternative initiation of translation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-CDC2L1 Pab (ABIN391737) is used in Western blot to detect CDC2L1 in CHO cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Cornelis, Bruynooghe, Denecker et al.: "Identification and characterization of a novel cell cycle-regulated internal ribosome entry site." in: Molecular cell, Vol. 5, Issue 4, pp. 597-605, 2000 (PubMed).

Hu, Mayeda, Trembley et al.: "CDK11 complexes promote pre-mRNA splicing." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8623-9, 2003 (PubMed).

Chen, Yin, Zhu et al.: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003 (PubMed).