Cyclin-Dependent Kinase 9 (CDK9) (C-Term) antibody

Antigen: Cyclin-Dependent Kinase 9 (CDK9)

Synonyms: TAK, C-2k, CTK1, CDC2L4, PITALRE, CDK9, P-TEF, P-TEFb, PTefb, DmelCG5179, CG5179, wu:fb22e09, wu:fc06c02, MGC95175, cdk9, MGC53831, MGC139578, tak, c-2k, cdc2l4, pitalre, MGC69296

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391760

Additional Information

Alternative name: CDK9

Gene ID: 1025

UniProt: P50750

Immunogen: This CDK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human CDK9.

Isotype: Ig

Description: Other names: Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, C-2K

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human CDK9.

Molecular Weight: 42791 DA

Comments:

Background: CKD9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-hCDK9-S357 Pab (ABIN391760) in HepG2, Jurkat (middle), and 294 cell line lysates. hCDK9-S357(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Western blot analysis of CDK9 (arrow) using rabbit polyclonal CDK9 Antibody (S357) (ABIN391760). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK9 gene.

Publications

Product: Graña, De Luca, Sang et al.: "PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3834-8, 1994 (PubMed).

Fu, Peng, Lee et al.: "Cyclin K functions as a CDK9 regulatory subunit and participates in RNA polymerase II transcription." in: The Journal of biological chemistry, Vol. 274, Issue 49, pp. 34527-30, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).