Aconitase 2, Mitochondrial (ACO2) (C-Term) antibody

Antigen: Aconitase 2, Mitochondrial (ACO2)

Synonyms: ACONM, MGC20605, MGC33908, Aco3, Aco-2, D10Wsu183e, cb1017, wu:fa10e03, wu:fb69g04, wu:fc20c11, MGC84375, aconitase 2, F10M23.310, F10M23_310

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391810

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ACO2 (C-term)

Gene ID: 50

Swiss-Prot: Q99798

Immunogen: This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633~662 amino acids from the C-terminal region of human ACO2.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Aconitate hydratase, mitochondrial [Precursor], Aconitase, Citrate hydro-lyase, Citrate hydro-lyase, ACO2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ACO2.

Molecular Weight: 85425 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody (C-term) (ABIN391810), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (C-term) (ABIN391810).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).

ACO2 Antibody (C-term) (ABIN391810) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ACO2 antibody detected the ACO2 protein (arrow).