anti-Cullin 4A antibody (CUL4A) (AA 102-131)

Details for Product anti-CUL4A Antibody No. ABIN391852
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AA 102-131, N-Term
(28), (15), (7), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
(82), (26), (19), (1)
(77), (5)
Clonality (Clone)
Polyclonal ()
This Cullin 4A antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(60), (33), (22), (12), (12), (9), (8), (3), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Immunogen This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 AA from the N-terminal region of human Cullin 4A (CUL4A).
Clone RB10557
Isotype Ig
Specificity This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human CUL4a.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background CUL4a is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
Synonyms: Cullin-4A, CUL-4A, CUL4A
Molecular Weight 87680 DA
Gene ID 8451
UniProt Q13619
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Cullin 4A antibody (CUL4A) (AA 102-131) (ABIN391852) Western blot analysis of CUL4a (arrow) using rabbit polyclonal CUL4a Antibody (Human ...
Background publications Hu, Zacharek, He et al.: "WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase." in: Genes & development, Vol. 22, Issue 7, pp. 866-71, 2008 (PubMed).

Huang, Chen: "VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation." in: Oncogene, Vol. 27, Issue 29, pp. 4056-64, 2008 (PubMed).

Wen, Duus, Friedrich et al.: "The HIV1 protein Vpr acts to promote G2 cell cycle arrest by engaging a DDB1 and Cullin4A-containing ubiquitin ligase complex using VprBP/DCAF1 as an adaptor." in: The Journal of biological chemistry, Vol. 282, Issue 37, pp. 27046-57, 2007 (PubMed).

Catalog No. ABIN391852
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