Ephrin Type A Receptor 1 (EPHA1) (N-Term), (AA 29-60) antibody

Details for Product No. ABIN391883
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Antigen
Synonyms EPH, EPHT, EPHT1, 5730453L17Rik, AL033318, Eph, Esk, EPHA9
Epitope
N-Term, AA 29-60
(16), (11), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(65), (16), (2), (1), (1), (1), (1)
Host
Rabbit
(58), (16), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(73), (52), (32), (15), (11), (10), (6), (5), (3), (3)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN391883
291.50 $
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Immunogen This EphA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-60 AA from the N-terminal region of human EphA1.
Clone RB1576
Isotype Ig
Specificity This EphA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~67 amino acids from the N-terminal region of human EphA1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EphA1
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Synonyms: Ephrin type-A receptor 1, Tyrosine-protein kinase receptor EPH
Molecular Weight 108080 DA
Gene ID 2041
UniProt P21709
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 1.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Ephrin Type A Receptor 1 (EPHA1) (N-Term), (AA 29-60) antibody Western blot analysis of anti-EphA1 N-term Pab (ABIN391883) in HeLa cell lysate. EphA1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Ephrin Type A Receptor 1 (EPHA1) (N-Term), (AA 29-60) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Ephrin Type A Receptor 1 (EPHA1) (N-Term), (AA 29-60) antibody (3) Flow cytometric analysis of HepG2 cells using EphA1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

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