Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (C-Term) antibody

Antigen: Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)

Synonyms: Flk1, Ly73, Flk-1, Krd-1, VEGFR2, VEGFR-2, sVEGFR-2, 6130401C07, FLK1, CD309, VEGFR, Vegfr-2, MGC93590, KDR, FLK-1, flk-1, vegfr-2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391979

Additional Information

Alternative name: VEGFR2 (FLK1/KDR)

Gene ID: 3791

UniProt: P35968

Immunogen: This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1327~1356 amino acids from the C-terminal region of human KDR/FLK1.

Isotype: Ig

Description: Other names: Vascular endothelial growth factor receptor 2, VEGFR-2, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, KDR, FLK1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1327~1356 amino acids from the C-terminal region of human KDR/FLK1.

Molecular Weight: 151526 DA

Comments:

Background: KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-KDR/FLK1 Pab (ABIN391979) in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Elvert, Kappel, Heidenreich et al.: "Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7520-30, 2003 (PubMed).

Kiba, Yabana, Shibuya: "A set of loop-1 and -3 structures in the novel vascular endothelial growth factor (VEGF) family member, VEGF-ENZ-7, is essential for the activation of VEGFR-2 signaling." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13453-61, 2003 (PubMed).

Zeng, Zhao, Yang et al.: "Heterotrimeric G alpha q/G alpha 11 proteins function upstream of vascular endothelial growth factor (VEGF) receptor-2 (KDR) phosphorylation in vascular permeability factor/VEGF signaling." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20738-45, 2003 (PubMed).

Yilmaz, Kliche, Mayr-Beyrle et al.: "p38 MAPK inhibition is critically involved in VEGFR-2-mediated endothelial cell survival." in: Biochemical and biophysical research communications, Vol. 306, Issue 3, pp. 730-6, 2003 (PubMed).