C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (N-Term) antibody

Antigen: C-Mer Proto-Oncogene Tyrosine Kinase (MERTK)

Synonyms: MER, RP38, c-mer, MGC133349, Eyk, Mer, Nyk, rdy, C-EYK, MERTK, DKFZp469D2215, si:ch1073-268l12.2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392017

Additional Information

Alternative name: MERTK

Gene ID: 10461

UniProt: Q12866

Immunogen: This MERTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~44 amino acids from the N-terminal region of human MERK.

Isotype: Ig

Description: Other names: Receptor tyrosine kinase MerTK, MERTK, MER

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MERTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~44 amino acids from the N-terminal region of human MERK.

Molecular Weight: 110390 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:10~50 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-MERK Pab (ABIN392017) in NIH/3T3 cell lysate. MERK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human brain tissue reacted with MERK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Graham, Dawson, Mullaney et al.: "Cloning and mRNA expression analysis of a novel human protooncogene, c-mer." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 647-57, 1994 (PubMed).

Thompson, McHenry, Li et al.: "Retinal dystrophy due to paternal isodisomy for chromosome 1 or chromosome 2, with homoallelism for mutations in RPE65 or MERTK, respectively." in: American journal of human genetics, Vol. 70, Issue 1, pp. 224-9, 2001 (PubMed).