Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (Ser767) antibody

Antigen: Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA)

Synonyms: CD140A, PDGFR2, MGC74795, RHEPDGFRA, PDGFRA

Binding Site: Ser767

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392026

Additional Information

Alternative name: PDGFRA

Gene ID: 5156

UniProt: NP_006197

Immunogen: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S767 of human PDGFRA.

Isotype: Ig

Description: Other names: PDGF-R-alpha, CD140a antigen, Alpha platelet-derived growth factor receptor

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S767 of human PDGFRA.

Molecular Weight: 122669 DA

Comments:

Background: PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of PDGFRA (arrow) using rabbit polyclonal PDGFRA Antibody (S767) (ABIN392026). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (S767) (ABIN392026), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Subramanian, West, Corless et al.: "Gastrointestinal stromal tumors (GISTs) with KIT and PDGFRA mutations have distinct gene expression profiles." in: Oncogene, Vol. 23, Issue 47, pp. 7780-90, 2004 (PubMed).

Sakurai, Hasegawa, Sakuma et al.: "Myxoid epithelioid gastrointestinal stromal tumor (GIST) with mast cell infiltrations: a subtype of GIST with mutations of platelet-derived growth factor receptor alpha gene." in: Human pathology, Vol. 35, Issue 10, pp. 1223-30, 2004 (PubMed).

Hiwatari, Taki, Tsuchida et al.: "Novel missense mutations in the tyrosine kinase domain of the platelet-derived growth factor receptor alpha(PDGFRA) gene in childhood acute myeloid leukemia with t(8;21)(q22;q22) or inv(16)(p13q22)." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 19, Issue 3, pp. 476-7, 2005 (PubMed).

Debiec-Rychter, Cools, Dumez et al.: "Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC412 inhibitor against imatinib-resistant mutants." in: Gastroenterology, Vol. 128, Issue 2, pp. 270-9, 2005 (PubMed).

Wilczynski, Chen, Chen et al.: "Expression and mutational analysis of tyrosine kinase receptors c-kit, PDGFRalpha, and PDGFRbeta in ovarian cancers." in: Human pathology, Vol. 36, Issue 3, pp. 242-9, 2005 (PubMed).