Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 732-761), (Tyr754) antibody

Details for Product No. ABIN392029
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Antigen
Synonyms AI115593, CD140a, Pdgfr-2, APDGFR, PDGFACE, CD140A, PDGFR-2, PDGFR2, RHEPDGFRA, PDGFR, PDGFR-A
Epitope
AA 732-761, Tyr754
(31), (23), (20), (19), (16), (13), (13), (12), (12), (12), (10), (9), (9), (9), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(285), (173), (143), (84), (72), (37), (36), (1)
Host
Rabbit
(264), (29), (19), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(21), (16), (14), (13), (12), (7), (7), (7), (7), (7), (7), (7), (7), (7), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(169), (123), (89), (70), (68), (35), (23), (18), (12), (6), (3), (3), (2), (2), (2)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN392029
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Immunogen This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 732-761 AA from human PDGFRA.
Clone RB11213
Isotype Ig
Specificity This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y754 of human PDGFRA.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PDGFRA
Background PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Synonyms: PDGF-R-alpha, CD140a antigen, Alpha platelet-derived growth factor receptor
Molecular Weight 122669 DA
Gene ID 5156
NCBI Accession NP_006197
Research Area Stem Cells, Protein Modifications, Cell Structure, Cell Cycle
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 732-761), (Tyr754) antibody Western blot analysis of PDGFRA (arrow) using rabbit polyclonal PDGFRA Antibody (Y754) (ABIN392029). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2) (Origene Technologies).
anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 732-761), (Tyr754) antibody (2) Immunofluorescence analysis of anti-PDGFRA polyclonal antibody (ABIN392029) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 732-761), (Tyr754) antibody (3) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y754), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Subramanian, West, Corless et al.: "Gastrointestinal stromal tumors (GISTs) with KIT and PDGFRA mutations have distinct gene expression profiles." in: Oncogene, Vol. 23, Issue 47, pp. 7780-90, 2004 (PubMed).

Sakurai, Hasegawa, Sakuma et al.: "Myxoid epithelioid gastrointestinal stromal tumor (GIST) with mast cell infiltrations: a subtype of GIST with mutations of platelet-derived growth factor receptor alpha gene." in: Human pathology, Vol. 35, Issue 10, pp. 1223-30, 2004 (PubMed).

Hiwatari, Taki, Tsuchida et al.: "Novel missense mutations in the tyrosine kinase domain of the platelet-derived growth factor receptor alpha(PDGFRA) gene in childhood acute myeloid leukemia with t(8;21)(q22;q22) or inv(16)(p13q22)." in: Leukemia, Vol. 19, Issue 3, pp. 476-7, 2005 (PubMed).

Debiec-Rychter, Cools, Dumez et al.: "Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC412 inhibitor against imatinib-resistant mutants." in: Gastroenterology, Vol. 128, Issue 2, pp. 270-9, 2005 (PubMed).

Wilczynski, Chen, Chen et al.: "Expression and mutational analysis of tyrosine kinase receptors c-kit, PDGFRalpha, and PDGFRbeta in ovarian cancers." in: Human pathology, Vol. 36, Issue 3, pp. 242-9, 2005 (PubMed).

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