Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term), (AA 9-39) antibody

Details for Product No. ABIN392070
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Antigen
Synonyms 4432404K01Rik, ARTC4, DO, DOK1, AW557123, p62DOK, Dok-1, P62DOK
Epitope
N-Term, AA 9-39
(40), (35), (13), (13), (13), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(193), (118), (118), (41), (41), (41)
Host
Rabbit
(192), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(143), (57), (44), (43), (41), (14), (12), (11), (4), (2), (2)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN392070
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Immunogen This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 AA from the N-terminal region of human DOK1.
Clone RB1759-RB1760
Isotype Ig
Specificity This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human DOK1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name DOK1
Background DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Synonyms: p62(dok), Downstream of tyrosine kinase 1, pp62
Molecular Weight 52391 DA
Gene ID 1796
UniProt Q99704
Research Area Stem Cells, Cell Structure, Cancer
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term), (AA 9-39) antibody Western blot analysis of anti-DOK1 Pab (ABIN392070) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term), (AA 9-39) antibody (2) Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term) (ABIN392070). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2).
anti-Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term), (AA 9-39) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Nelms, Snow, Noben-Trauth: "Dok1 encoding p62(dok) maps to mouse chromosome 6 and human chromosome 2 in a region of translocation in chronic lymphocytic leukemia." in: Genomics, Vol. 53, Issue 2, pp. 243-5, 1998 (PubMed).

Némorin, Duplay: "Evidence that Llck-mediated phosphorylation of p56dok and p62dok may play a role in CD2 signaling." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14590-7, 2000 (PubMed).

Hubert, Ferreira, Debre et al.: "Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del)." in: European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics, Vol. 27, Issue 3, pp. 145-8, 2000 (PubMed).

Liang, Wisniewski, Strife et al.: "Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13732-8, 2002 (PubMed).

Yamakawa, Tsuchida, Sugino: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." in: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, 2002 (PubMed).

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