DOK2 (N-Term) antibody

Antigen: DOK2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392071

Additional Information

Gene ID: 9046

UniProt: O60496

Immunogen: This DOK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human DOK2.

Isotype: Ig

Description: Other names: p56(dok-2), Docking protein 2, Downstream of tyrosine kinase 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DOK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human DOK2.

Molecular Weight: 45548 DA

Comments:

Background: Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. Dok-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. Dok-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the Dok1 and Dok2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the Dok proteins serve as mediators of bcr-abl signaling. The role of Dok proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9,22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Signaling, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-DOK2 Pab (ABIN392071) in mouse spleen tissue line lysate (35ug/lane). DOK2(arrow) was detected using the purified Pab.

Western blot analysis of DOK2 (arrow) using DOK2 Antibody (N-term) (ABIN392071). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Di Cristofano, Carpino, Dunant et al.: "Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4827-30, 1998 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).