tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 10-39), (N-Term) antibody

Details for Product No. ABIN392082, Supplier: Log in to see
Antigen
  • ACK
  • ACK-1
  • ACK1
  • p21cdc42Hs
  • AW552477
  • Ack
  • Cdgip
  • Pyk1
  • tyrosine kinase, non-receptor, 2
  • TNK2
  • Tnk2
  • LOC100350340
Epitope
AA 10-39, N-Term
36
21
18
16
16
15
12
11
9
8
8
7
6
6
5
5
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
184
79
63
19
3
3
3
Host
Rabbit
184
21
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
13
13
11
10
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
139
116
42
34
27
26
22
8
6
5
4
3
3
1
Options
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Immunogen This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 AA from the N-terminal region of human ACK1.
Clone RB15601
Isotype Ig
Specificity This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human ACK1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ACK1 (TNK2 Antibody Abstract)
Background ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.
Synonyms: Tyrosine kinase non-receptor protein 2, Activated CDC42 kinase 1, ACK-1, ACK1, TNK2
Molecular Weight 114327 DA
Gene ID 10188
UniProt Q07912
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 10-39), (N-Term) antibody (ABIN392082) Western blot analysis of ACK1 Antibody (N-term) (ABIN392082) in K562 cell line lysate...
Western Blotting (WB) image for anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 10-39), (N-Term) antibody (ABIN392082) Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term) ...
Immunohistochemistry (IHC) image for anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 10-39), (N-Term) antibody (ABIN392082) Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody ...
Background publications Yokoyama, Miller: "Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 47713-23, 2003 (PubMed).

Manser, Leung, Salihuddin, Tan, Lim: "A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42." in: Nature, Vol. 363, Issue 6427, pp. 364-7, 1993 (PubMed).

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