tyrosine Kinase, Non-Receptor, 2 (TNK2) (Tyr80) antibody

Antigen: tyrosine Kinase, Non-Receptor, 2 (TNK2)

Binding Site: Tyr80

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392086

Additional Information

Alternative name: ACK1

Gene ID: 10188

UniProt: Q07912

Immunogen: This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 814~844 amino acids surrounding Y826 of human ACK1.

Isotype: Ig

Description: Other names: Tyrosine kinase non-receptor protein 2, Activated CDC42 kinase 1, ACK-1, ACK1, TNK2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 814~844 amino acids surrounding Y826 of human ACK1.

Molecular Weight: 114327 DA

Comments:

Background: ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of ACK1 (ABIN392086) in Y79 cell line lysates (35ug/lane).ACK1 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of TNK2(arrow) using rabbit polyclonal ACK1 (ABIN392086). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TNK2 gene (Lane 2) (Origene Technologies).

Publications

Product: Manser, Leung, Salihuddin et al.: "A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42." in: Nature, Vol. 363, Issue 6427, pp. 364-7, 1993 (PubMed).

Yokoyama, Miller: "Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 47713-23, 2003 (PubMed).