Activin Receptor Type IA (ACVR1) (Center,Arg147) antibody

Antigen: Activin Receptor Type IA (ACVR1)

Binding Site: Center,Arg147

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392240

Additional Information

Gene ID: 90

UniProt: Q04771

Immunogen: This ACVR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the center region of human ACVR1.

Isotype: Ig

Description: Other names: Activin receptor type I, ACTR-I, Serine/threonine-protein kinase receptor R1, SKR1, Activin receptor-like kinase 2, ALK-2, TGF-B superfamily receptor type I, TSR-I, ACVRLK2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACVR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the center region of human ACVR1.

Molecular Weight: 57153 DA

Comments:

Background: Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. ACVR1 (activin A type I receptor) signals a particular transcriptional response in concert with activin type II receptors.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

The anti-ACVR1 Pab (ABIN392240) is used in Western blot to detect ACVR1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Western blot analysis of ACVR1 (arrow) using rabbit polyclonal ACVR1 Antibody (R147) (ABIN392240). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACVR1 gene.

Publications

Product: Matsuzaki, Xu, Wang et al.: "A widely expressed transmembrane serine/threonine kinase that does not bind activin, inhibin, transforming growth factor beta, or bone morphogenic factor." in: The Journal of biological chemistry, Vol. 268, Issue 17, pp. 12719-23, 1993 (PubMed).

ten Dijke, Ichijo, Franzén et al.: "Activin receptor-like kinases: a novel subclass of cell-surface receptors with predicted serine/threonine kinase activity." in: Oncogene, Vol. 8, Issue 10, pp. 2879-87, 1993 (PubMed).