Activin A Receptor Type II-Like 1 (ACVRL1) (C-Term) antibody

Antigen: Activin A Receptor Type II-Like 1 (ACVRL1)

Synonyms: FOP, ALK2, SKR1, TSRI, ACTRI, ACVR1A, ACVRLK2, HHT, ALK1, HHT2, ORW2, SKR3, ALK-1, TSR-I, ACVRLK1, R3, R-3, SETHKIR, MGC91691, ACVRL1, MGC139457, DKFZp459C148, vgb, alk1, zehn1109, hm:zehn1109

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392242

Additional Information

Alternative name: ACVRL1

Gene ID: 94

UniProt: P37023

Immunogen: This ACVRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474~503 amino acids from the C-terminal region of human ACVRL1.

Isotype: Ig

Description: Other names: SKR3, Activin receptor-like kinase 1, ALK-1, TGF-B superfamily receptor type I, TSR-I

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACVRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474~503 amino acids from the C-terminal region of human ACVRL1.

Molecular Weight: 56124 DA

Comments:

Background: ACVRL1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. This protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of hACVRL1-L489 (ABIN392242) in Jurkat, HepG2 cell line lysates (35ug/lane). ACVRL1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

ACVRL1 Antibody (C-term) (ABIN392242) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Attisano, Cárcamo, Ventura et al.: "Identification of human activin and TGF beta type I receptors that form heteromeric kinase complexes with type II receptors." in: Cell, Vol. 75, Issue 4, pp. 671-80, 1993 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).