V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (Thr400) antibody

Antigen: V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF)

Synonyms: BRAF1, RAFB1, B-RAF1, FLJ95109, MGC126806, MGC138284, RMIL, C-RMIL, B-Raf, braf1, rafb1, b-raf1, BRAF

Binding Site: Thr400

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392248

Additional Information

Alternative name: BRAF

Gene ID: 673

UniProt: P15056

Immunogen: This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.

Isotype: Ig

Description: Other names: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.

Molecular Weight: 84490 DA

Comments:

Background: BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Application Details

Application Notes: The suggested dilution is:

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T400) (ABIN392248). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF-pT400, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).

Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).

Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).