|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Small Nuclear Ribonucleoprotein Polypeptide E (SNRPE) (Thr439) antibody
|Synonyms||SME, Sm-E, B-raf|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T439 of human BRAF.|
|Description||Other names: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T439 of human BRAF.|
|Molecular Weight||84490 DA|
Background: BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting ~~ 1:50~100 Immunohistochemistry ~~ 1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology|
|Restrictions||For Research Use only|
Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).
Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).
Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).