Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (C-Term) antibody

Antigen: Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1)

Synonyms: RIP, RIP1, FLJ39204, Rinp, Rip1, D330015H01Rik

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392273

Additional Information

Alternative name: RIPK1

Gene ID: 8737

UniProt: NP_003795

Immunogen: This RIPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585~671 amino acids from the C-terminal region of human RIPK1.

Isotype: Ig

Description: Other names: Receptor-interacting serine/threonine protein kinase 2, Serine/threonine protein kinase RIP, Cell death protein RIP, Receptor interacting protein, RIP

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RIPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585~671 amino acids from the C-terminal region of human RIPK1.

Molecular Weight: 75958 DA

Comments:

Background: RIPK1 is a serine/threonine kinase that promotes apoptosis and activation of NF-kappa-B. This protein is required for TNFRSF1A mediated activation of NF-kappa-B. It is proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Immunohistochemistry~~1:50~100 Dot Blot ~~ 1:500 Western Blot~~ 1:250~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hRIPK1-K585 (ABIN392273) in Jurkat and K562 cell line lysates (35ug/lane). RIPK1 (arrow) was detected using the purified Pab.

Western blot analysis of anti-RIPK1 Pab (ABIN392273) transiently transfected HEK-293 cell line lysate (1ug/lane). RIPK1 pab(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with RIPK1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).