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RIPK3 antibody (Receptor-Interacting serine-threonine Kinase 3) (AA 489-518)

Details for Product anti-RIPK3 Antibody No. ABIN392275, Supplier: Log in to see
Antigen
  • 2610528K09Rik
  • AW107945
  • Hcyp2
  • Rip3
  • RIP3
  • RIPK3
Epitope
AA 489-518, C-Term
31
20
16
15
10
7
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
121
41
18
7
6
4
3
2
1
Host
Rabbit
128
24
Clonality (Clone)
Polyclonal ()
Conjugate
This RIPK3 antibody is un-conjugated
9
7
5
5
5
5
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
137
65
62
32
8
6
6
4
2
1
Supplier
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Immunogen This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 AA from the C-terminal region of human RIPK3.
Clone RB3319
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name RIPK3 (RIPK3 Antibody Abstract)
Background RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Synonyms: Receptor-interacting serine/threonine protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, RIP3
Molecular Weight 56901 Da
Gene ID 11035
NCBI Accession NP_006862
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-RIPK3 antibody (Receptor-Interacting serine-threonine Kinase 3) (AA 489-518) (ABIN392275) Western blot analysis of hRIPK3-E504 (ABIN392275) in K562 cell line lysates (35 µg/la...
Western Blotting (WB) image for anti-RIPK3 antibody (Receptor-Interacting serine-threonine Kinase 3) (AA 489-518) (ABIN392275) Western blot analysis of hRIPK3-E504 (ABIN392275) in mouse cerebellum tissue lysates ...
Immunohistochemistry (IHC) image for anti-RIPK3 antibody (Receptor-Interacting serine-threonine Kinase 3) (AA 489-518) (ABIN392275) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (R...
Product cited in: Deutsch, Graffeo, Rokosh, Pansari, Ochi, Levie, Van Heerden, Tippens, Greco, Barilla, Tomkötter, Zambirinis, Avanzi, Gulati, Pachter, Torres-Hernandez, Eisenthal, Daley, Miller: "Divergent effects of RIP1 or RIP3 blockade in murine models of acute liver injury." in: Cell death & disease, Vol. 6, pp. e1759, 2015 (PubMed).

Dara, Johnson, Suda, Win, Gaarde, Han, Kaplowitz: "Receptor interacting protein kinase 1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis." in: Hepatology (Baltimore, Md.), 2015 (PubMed).

Roychowdhury, Chiang, Mandal, McMullen, Liu, Cohen, Pollard, Feldstein, Nagy: "Inhibition of apoptosis protects mice from ethanol-mediated acceleration of early markers of CCl4 -induced fibrosis but not steatosis or inflammation." in: Alcoholism, clinical and experimental research, Vol. 36, Issue 7, pp. 1139-47, 2012 (PubMed).

Zorde-Khvalevsky, Abramovitch, Barash, Spivak-Pohis, Rivkin, Rachmilewitz, Galun, Giladi: "Toll-like receptor 3 signaling attenuates liver regeneration." in: Hepatology (Baltimore, Md.), Vol. 50, Issue 1, pp. 198-206, 2009 (PubMed).

Background publications Sun, Lee, Navas, Baldwin, Stewart, Dixit: "RIP3, a novel apoptosis-inducing kinase." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 16871-5, 1999 (PubMed).