Testis-Specific Kinase 1 (TESK1) (C-Term) antibody

Antigen: Testis-Specific Kinase 1 (TESK1)

Synonyms: TSSK1, FKSG81, SPOGA4, STK22D, TESK1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392276

Additional Information

Alternative name: TESK1

Gene ID: 7016

UniProt: Q15569

Immunogen: This TESK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446~476 amino acids from the C-terminal region of human TESK1.

Isotype: Ig

Description: Other names: Testis-specific protein kinase 1, Testicular protein kinase 1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TESK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446~476 amino acids from the C-terminal region of human TESK1.

Molecular Weight: 67712 DA

Comments:

Background: TESK1 is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases(LIMKs). TESK1 can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that TESK1 plays an important role at and after the meiotic phase of spermatogenesis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TESK1 Antibody (C-term) (ABIN392276) in A375 cell lysate. TESK1 (arrow) was detected using purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Toshima, Ohashi, Okano et al.: "Identification and characterization of a novel protein kinase, TESK1, specifically expressed in testicular germ cells." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31331-7, 1996 (PubMed).