zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Center) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392281

Additional Information

Alternative name: ZAP70

Gene ID: 7535

UniProt: P43403

Immunogen: This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids from the center region of human ZAP70.

Isotype: Ig

Description: Other names: Tyrosine-protein kinase ZAP-70, 70 kDa zeta-associated protein, Syk-related tyrosine kinase

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids from the center region of human ZAP70.

Molecular Weight: 69872 DA

Comments:

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

ZAP70 Antibody (A267) (ABIN392281) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow).

Publications

Product: Wiestner, Rosenwald, Barry et al.: "ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile." in: Blood, Vol. 101, Issue 12, pp. 4944-51, 2003 (PubMed).

Kremer, Humphreys, Kumar et al.: "Distinct role of ZAP-70 and Src homology 2 domain-containing leukocyte protein of 76 kDa in the prolonged activation of extracellular signal-regulated protein kinase by the stromal cell-derived factor-1 alpha/CXCL12 chemokine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 1, pp. 360-7, 2003 (PubMed).

Kuhné, Lin, Yablonski et al.: "Linker for activation of T cells, zeta-associated protein-70, and Src homology 2 domain-containing leukocyte protein-76 are required for TCR-induced microtubule-organizing center polarization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 860-6, 2003 (PubMed).

Yang, Villain, Mustelin et al.: "Critical role of Ser-520 phosphorylation for membrane recruitment and activation of the ZAP-70 tyrosine kinase in T cells." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7667-77, 2003 (PubMed).

Kuroyama, Ikeda, Kasai et al.: "Identification of a novel isoform of ZAP-70, truncated ZAP kinase." in: Biochemical and biophysical research communications, Vol. 315, Issue 4, pp. 935-41, 2004 (PubMed).