COP9 Constitutive Photomorphogenic Homolog Subunit 3 (Arabidopsis) (COPS3) (C-Term) antibody

Antigen: COP9 Constitutive Photomorphogenic Homolog Subunit 3 (Arabidopsis) (COPS3)

Synonyms: CSN3, SGN3, MGC94156, COPS3, MGC166268, DKFZp468P1922, Csn3, Sgn3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392340

Additional Information

Alternative name: COPS3

Gene ID: 8533

UniProt: Q9UNS2

Immunogen: This COPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345~375 amino acids from the C-terminal region of human COPS3.

Isotype: Ig

Description: Other names: COP9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3, CSN3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This COPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345~375 amino acids from the C-terminal region of human COPS3.

Molecular Weight: 47873 DA

Comments:

Background: COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. This protein phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-COPS3 Antibody (C-term) (ABIN392340) in Ramos cell line lysates (35ug/lane). COPS3(arrow) was detected using the purified Pab.

Western blot analysis of COPS3 (arrow) using rabbit polyclonal COPS3 Antibody (C-term) (ABIN392340). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COPS3 gene.

Publications

Product: Potocki, Chen, Lupski: "Subunit 3 of the COP9 signal transduction complex is conserved from plants to humans and maps within the smith-magenis syndrome critical region in 17p11.2." in: Genomics, Vol. 57, Issue 1, pp. 180-2, 1999 (PubMed).

Bech-Otschir, Kraft, Huang et al.: "COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system." in: The EMBO journal, Vol. 20, Issue 7, pp. 1630-9, 2001 (PubMed).

Henriksen, Aagesen, Maelandsmo et al.: "Amplification and overexpression of COPS3 in osteosarcomas potentially target TP53 for proteasome-mediated degradation." in: Oncogene, Vol. 22, Issue 34, pp. 5358-61, 2003 (PubMed).