Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) (N-Term) antibody

Antigen: Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)

Synonyms: ATQ, wu:fi34d12, wu:fi35e06, atq1, ALDH7A1, MGC128050, antiquitin, Atq1, D18Wsu181e, EPD, PDE, ATQ1, FLJ11738, FLJ92814, MGC83352

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392358

Additional Information

Alternative name: ALDH7A1

Gene ID: 501

UniProt: NP_001173

Immunogen: This ALDH7A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~69 amino acids from the N-terminal region of human ALDH7A1.

Isotype: Ig

Description: Other names: Antiquitin-1, Aldehyde dehydrogenase family 7 member A1, Delta1-piperideine-6-carboxylate dehydrogenease, P6c dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Short=Alpha-AASA dehydrogenase, ALDH7A1, ATQ1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ALDH7A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~69 amino acids from the N-terminal region of human ALDH7A1.

Molecular Weight: 55366 DA

Comments:

Background: Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in ALDH7A1 gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ALDH7A1 Antibody (N-term) (ABIN392358) in HepG2 cell line lysates (35ug/lane). ALDH7A1(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH7A1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ALDH7A1 Antibody (N-term) (ABIN392358) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Kanno, Kure, Narisawa et al.: "Allelic and non-allelic heterogeneities in pyridoxine dependent seizures revealed by ALDH7A1 mutational analysis." in: Molecular genetics and metabolism, Vol. 91, Issue 4, pp. 384-9, 2007 (PubMed).